Research Topic · Peer-Reviewed

MTT Assay

The MTT assay is a widely used colorimetric method for measuring cell viability and metabolic activity. It is based on the conversion of the yellow tetrazolium dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an insoluble purple formazan product by enzymes in metabolically active cells. Af…

Curated from this journal's research 📚 12 peer-reviewed articles cited Cited 62× across the literature 🗓 Reviewed June 2026

Overview

The MTT assay is a widely used colorimetric method for measuring cell viability and metabolic activity. It is based on the conversion of the yellow tetrazolium dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an insoluble purple formazan product by enzymes in metabolically active cells. After the formazan is dissolved, its concentration is measured by absorbance and reflects the number of viable cells, allowing researchers to quantify how cells respond to drugs, toxins, nanoparticles, and other treatments in vitro. The MTT assay is part of the scope of Advanced Cytology, which studies cells and their responses under experimental conditions. Research in this area applies cell-viability and cytotoxicity measurement across many cell types and treatments, including the cytotoxic and apoptotic activity of silver nanoparticles on cultured cancer cell lines, in vitro assessment of cardiac function using a rat cardiomyocyte cell line, and evaluation of effects on cell-based biomarkers in cultured cells. These studies show how the assay supports drug screening, toxicity testing, and the broader investigation of cell health. This page gathers peer-reviewed, open-access research relevant to the MTT assay and cell-viability analysis.

Research published in this journal

12 peer-reviewed articles, ranked by relevance. Each links to its DOI.

How this research is being cited

The 12 articles above have been cited 62 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.

A sample of recent works citing this journal's research on MTT Assay, linking to each citing work.

Editorial oversight

Curated from peer-reviewed research published in Advanced Cytology.

Journal editorial board
Krzysztof Marycz · Poland MARIA VIOLETTA BRUNDO · Italy

This page summarises published research for orientation; it is not medical or professional advice.